How Substrate Concentration Affects Enzyme Activity Lab Report

How Subst lay out Concentration Affects Enzyme Activity The purpose of the sample was to observe how substratum density affects enzyme activity. My supposition is that the much substrate concentration thither is, the more(prenominal) p terminaluct it will kind; this is until it reaches the point of saturation, after(prenominal) that, the enzyme will be unable to produce at a faster rate. To prove this, twenty atomic depend 23 streaks were made, five trials for five different concentrations of substrate with the very(prenominal) amount of enzyme, recording the rate of intersection for each trial. For this experiment, henry peroxide was used as the substrate, and catalase as the enzyme. Each trial was five milliliters of substrate, adding ten drops of enzyme, delay 30 secs, shaking it twice, and burbly it into a bottle where the atomic number 8 (as the product) would be recorded. The outcome of this experiment turned out as follows: the come rate of molecular type O produced for two per centum henry peroxide was 0.05 percent of molecular oxygen per second; for four, six, octette and ten percent the average rate was 0.06, 0.07, 0.08, 0.10 percent of molecular oxygen per second respectively. The results are close to being linear, with an r² value of 0.9888. One could say the outcome is safe because it shows how by having more concentration of substrate the molecular oxygen production is increased. Alas, the investigative question cannot be fully answered; this is because more tests could throw away been made with higher concentration of substrate, showing a more accurate representation of what might happen, and, possibly, showing when increase the concentration the reaction rate would no longer increase, proving the hypothesis right. In addition, looking at the graph it is noticeable how the misplay disallow are ample; the reason for vast misplay debar could be caused by interrupting the experimental process by woful the table whil e the experiment was ongoing, inaccurate me! asuring rod of catalase enzyme, or cleanliness. In...If you want to get a full essay, rate it on our website: OrderEssay.net

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